Bioconjugation of Carbon Nanotubes – Overview and Methods
We discuss different methods for the bioconjugation of carbon nanotubes including amine, aldehyde and carboxyl based methods.
Methods for Protein and Antibody Bioconjugation to Gold
We discuss methods for protein and antibody bioconjugation to gold including theory, alternative approaches, and protocols.
Protein Labeling with Fluorescent Probes – Theory and Methods
We discuss types of fluorescent probes, how to select a fluorescent probe for protein labeling, protein labeling kits, and protocols…
Biopolymer surface functionalization: Simple Step-By-Step guide
Introduction to Surface Functionalization of Biopolymers Immobilizing (or covalently attaching) proteins, lipids, carbohydrates, and other polymers on biopolymer surfaces is…
Protein Purification of Recombinant Proteins
Protein Purification Summary In our previous blog posts we have explored Gene cloning with Plasmid Vectors in Bacteria, Transient transfection…
Calcium Phosphate Transient Transfection Protocol & Guide
Transfection with Calcium Phosphate: General Summary Molecular biology tools allow us to understand and manipulate DNA/RNA so that we can…
Immunofluorescence Microscopy Protocol and Method Guide
Immunofluorescence Microscopy Overview & Theory Immunofluorescence Microscopy (IF) is a classical technique to observe the localization of molecules in cell/tissue…
Immunoprecipitation (IP) Scientific Method Guide
Immunoprecipitation Overview Immunoprecipitation is a method for extracting protein from a solution. Typically, this solution is a cell lysate which…
Western Blot Theory and Method Guide
Overview of Western Blot Method A western blot enables sensitive detection of specific proteins from a solution containing multiple proteins.…
HPLC: Biochemical Analysis. A Step-By-Step Method Guide
HPLC Method Overview HPLC, or high performance liquid chromatography is an amazing analytical technique for chemical compounds including biopolymers, small…