Protein Purification of Recombinant Proteins
Protein Purification Summary In our previous blog posts we have explored Gene cloning with Plasmid Vectors in Bacteria, Transient transfection…
Gene Cloning using Plasmids: Molecular Cloning Intro
Gene Cloning with Plasmids: Summary We all know that DNA is the basic building block of biology. So, how can…
Bacterial Transformation Protocol with Competent Cells
Bacterial Transformation using Competent Cells: Summary Since we have already learned Calcium Phosphate Transfection with mammalian cells, let’s now focus…
Guide: Measure Cell Proliferation with Thymidine and BrdU
Thymidine and BrdU, Cell Proliferation Assay Summary How do you know if your cultured cells are growing? Does your new…
Cytotoxicity & Cell Viability with MTT Assay Protocol
Cell Viability with MTT Assay Summary Cell Viability is a common technique used by biochemists who are studying oncology and…
Calcium Phosphate Transient Transfection Protocol & Guide
Transfection with Calcium Phosphate: General Summary Molecular biology tools allow us to understand and manipulate DNA/RNA so that we can…
RNA Extraction, Isolation, and Purification by SciGine
RNA Extraction: General Summary RNA extraction and isolation is a precursor for many methods in molecular biology including Northern Blotting,…
Immunofluorescence Microscopy Protocol and Method Guide
Immunofluorescence Microscopy Overview & Theory Immunofluorescence Microscopy (IF) is a classical technique to observe the localization of molecules in cell/tissue…
Immunoprecipitation (IP) Scientific Method Guide
Immunoprecipitation Overview Immunoprecipitation is a method for extracting protein from a solution. Typically, this solution is a cell lysate which…
Southern Blot Scientific Method Theory and Guide
Southern Blots: Overview & Theory Southern Blotting is a technique that is used to detect whether you have a specific…