Bioconjugation of Carbon Nanotubes – Overview and Methods
We discuss different methods for the bioconjugation of carbon nanotubes including amine, aldehyde and carboxyl based methods.
Orthogonal Bioconjugation Techniques – Step by Step Methods, and Impact
We discuss orthogonal bioconjugation techniques and methods such as SPAAC & tetrazine ligation, as well as applications of bioorthogonal chemistry.
Ligate Sticky Ends via DNA Ligation
Ligation of Sticky Ends, Summary of DNA ligation We have already discussed a high level view of gene cloning in…
Gene Cloning using Plasmids: Molecular Cloning Intro
Gene Cloning with Plasmids: Summary We all know that DNA is the basic building block of biology. So, how can…
Bacterial Transformation Protocol with Competent Cells
Bacterial Transformation using Competent Cells: Summary Since we have already learned Calcium Phosphate Transfection with mammalian cells, let’s now focus…
Calcium Phosphate Transient Transfection Protocol & Guide
Transfection with Calcium Phosphate: General Summary Molecular biology tools allow us to understand and manipulate DNA/RNA so that we can…
RNA Extraction, Isolation, and Purification by SciGine
RNA Extraction: General Summary RNA extraction and isolation is a precursor for many methods in molecular biology including Northern Blotting,…
Immunoprecipitation (IP) Scientific Method Guide
Immunoprecipitation Overview Immunoprecipitation is a method for extracting protein from a solution. Typically, this solution is a cell lysate which…
Southern Blot Scientific Method Theory and Guide
Southern Blots: Overview & Theory Southern Blotting is a technique that is used to detect whether you have a specific…
HPLC: Biochemical Analysis. A Step-By-Step Method Guide
HPLC Method Overview HPLC, or high performance liquid chromatography is an amazing analytical technique for chemical compounds including biopolymers, small…